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https://repositorio.unichristus.edu.br/jspui/handle/123456789/1834| Title: | ANÁLISE MICROBIOLÓGICA DE LESÕES DE OSTEONECROSE DOS MAXILARES EM ALVÉOLOS DENTÁRIOS DE RATOS TRATADOS COM ÁCIDO ZOLEDRÔNICO |
| Authors: | Gomes, Maria Clara Mendes |
| metadata.dc.contributor.advisor: | Verde, Maria Elisa Quezado Lima |
| Issue Date: | 2024-12-07 |
| Abstract: | Aminobisfosfonatos são fármacos amplamente utilizados no tratamento de desordens osseodegenerativas e metástases ósseas. Um dos principais efeitos adversos da terapia com bisfosfonatos é a osteonecrose dos maxilares (OM). Este trabalho objetiva realizar uma análise microbiológica de lesões de osteonecrose em alvéolos de ratos que foram tratados com ácido zoledrônico. Foram utilizados ratos machos da linhagem Wistar (rattus novergicus)(n=8/grupo) para realização do protocolo. Três administrações semanais (Dias 0, 7, 14) de AZ 0,2 mg/kg ou solução salina, foram realizadas. No dia 42, os animais foram anestesiados (quetamina 80 mg/kg e xilazina 20 mg/kg por via intraperitoneal) e submetidos a exodontia do primeiro molar superior esquerdo. Posteriormente, os animais nos dias 42 (imediatamente após a exodontia), 44, 46 e 48 foram anestesiados e realizadas as coletas microbiológicas. No dia 49, uma administração adicional do tratamento sistêmico (AZ ou solução salina) foi realizada e, no dia 70, os animais foram eutanasiados por sobredose anestésica (3x a dose utilizada para anestesia) para a obtenção de hemimaxilas e posteriores análises radiográfica.Os dados foram expressos em forma de média e erro padrão, submetidoso ao teste de normalidade de Shapiro-Wilk e comparados por meio dos testes t de Student ou Anova para medidas repetidas seguido do pós teste de Bonferroni. Após analise não foram achadas diferenças significativas no tempo cirúrgico entre os grupos (p=0,395) da sobre o número de fraturas (p=0,791) o peso dos dentes (p=0,795) e sobre a área radiolúcida dos alvéolos (p=0,519), além de não obter diferenças da massa corpórea nos grupos salina (p=0,059) e o no grupo AZ (p=0,087).Em relação as coletas microbiológicas não houve diferenças significativas nas coletas 01 (p= 0,863); coleta 02 (p=0,677) e na coleta 03 (p=0,970). havendo apenas na coleta 04 (p=0,000). A análise das colônias revelou uma diversidade significativa de microrganismos, incluindo tanto fungos na forma de levedura quanto diferentes tipos de bactérias. Desta forma o presente estudo reforça a complexidade e a variabilidade de microrganismos associadas à OM. Entretanto estudos futuros, focados na análise específica dessa microbiota oral são essenciais, para possibilitar a determinação dos possíveis agentes causadores, bem como para desenvolvimento de tratamentos mais direcionados. |
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| Keywords: | ácido zoledrônico arcada osseodentária microbiota bisfosfonatos |
| URI: | https://repositorio.unichristus.edu.br/jspui/handle/123456789/1834 |
| Appears in Collections: | Odontologia - Trabalhos de Conclusão de Curso - Campus PARQUE ECOLÓGICO |
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